ABSTRACT
Objective To analyze the genetic diversity of Aconitum brachypodum, an endangered medicinal plants. Methods Three pair primers were used to evaluate the relevant genetic parameters and clustering pattern for four natural populations containing 105 individuals by using POPGENE32, MEGA4, NTSYS and other biometric softwares. Results For A. brachypodum, the Nei’s genetic diversity index (He), Shannon’s information index (I), percentage of polymorphic loci (PPB) were 0.322 9 ± 0.179 8, 0.472 0 ± 0.251 7, and 80.57%, respectively; and the population level were 0.115 4 ± 0.044, 0.168 0 ± 0.065 3, and 12%, respectively. The genetic differentiation index (Gst) was 0.864 2. The clustering results showed that, Luquan and Dongchuan populations are grouped together, Lijiang and Huize populations are clustered together. Conclusion A.brachypodum possesses the relatively high level of genetic diversity at the species level, however, low within populations. This study should provide an initial insights for A.brachypodum germplasm conservation
ABSTRACT
OBJECTIVE: To investigate the clinical manifestations,chest imaging characteristics and treatment method of pneumonia and pleural effusion resulted from brucellosis. METHODS: The clinical data of a patient who suffered from pneumonia and pleural effusion resulted from brucellosis was collected and analyzed. RESULTS: The patient suffered from pneumonia and pleural effusion because of inhalation of aerosols containing brucella when handling apoblema of sick pigs.The main clinical manifestations included fever,chills,mild cough,mild sputum production,combined with chest congestion and shortness of breath. Chest imaging features were multiple patchy infiltrating shadows and nodules on the right lung combined with right side pleural effusion. Serum rose-bengal plate test was positive, and standard-tube agglutination test resulted in titres of 1 ∶ 200. After combination treatment using oral doxycycline plus rifampicin and intravenous ceftriaxone,the clinical symptom of the patient was relieved,chest patch shadows and pleural effusion were improved. CONCLUSION: Brucellosis with pulmonary involvement is rarely seen. Its clinical manifestations are diverse and non-specific. Pulmonary brucellosis could be easily misdiagnosed as common bacterial pneumonia or tuberculosis.Epidemiological data and occupational exposure history can help the diagnosis.